国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

簡要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:2263

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
把腿抬高我要添你下面小说| AV无码专区| 污视频在线观看| 亚洲精品日韩片无码中文字幕| 久久天天躁狠狠躁夜夜免费观看| japanese50mature日本亂倫| 亚洲AV产在线精品亚洲第一站| 人妻少妇屁股翘水多| 韩国三级| 精品无码人妻一区二区免费蜜桃 | 成人亚洲a片v一区二区三区蜜月| 久久久亚洲一区二区三区| 国产香蕉97碰碰久久人人| 中文字幕日韩欧美一区二区三区| 性生片30分钟| 亚洲av成人片无码www| 乌克兰少妇XXXX做受野外| 色婷婷一区二区三区四区成人网| 女人张开腿让男人添| 国产午夜三级一区二区三| 亚洲AV成人无码久久精品老人| 精品一区二区三区在线视频| 男女做爰猛烈动高潮a片免费应用| 黄色视频app下载| 亚洲人成亚洲人成在线观看| 全免费A级毛片免费看视频| 久久久久久精品免费免费自慰| 丁香五月天缴情在线| 一天不日b浑身难受| 男男黄gay片免费网站www| 国产SUV精二区69| 久久久久久九九99精品| 久久久久久精品一区二区三区日本| 精品无码久久久久久久久水蜜桃| gogo大胆欧美人体艺杧图片 | 国产白嫩护士被弄高潮| 揉她小豆豆揉到失禁h| 欧美特黄a级高清免费大片a片| 最近国语视频在线观看免费播放| sm调教贱屁股眼哭叫求饶h| 欧美裸体XXXX极品少妇|