国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

簡要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:2263

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国内精品伊人久久久久影院对白 | 成人性生交大片免费看京东小视频| 高h小月被几个老头调教| 阿娇被躁120分钟视频| 亚洲成A人片在线观看无码专区| 97视频在线观看| 无码人妻一区二区三区| 中文字幕亚洲欧美日韩在线不卡| 国产视频在线观看| 伸进内衣揉捏她的乳尖| 精品久久人人妻人人做精品| 亚洲AV午夜福利精品一区| 小受被多男摁住灌浓精a片小说| 久久久精品国产SM调教网站| 精品国产精品久久一区免费式| 日本真人做爰免费视频120秒| 新婚娇妻陪局长出差bd| 日本人妻丰满熟妇久久久久久| 亚洲 欧美 国产 日韩 精品 | 欧美老妇疯狂XXXXBBBB| 校花张开腿疯狂娇吟k视频| 全是肉的高h短篇bl| 国产乱人伦真实精品视频| 欧美性大战XXXXX久久久√| 揉腿却揉到两腿之间是湿的 | 乱人伦人妻精品一区二区 | 特级毛片爽WWW免费版| 再用点力今晚随你弄| 公开高潮当众露出羞耻h| 男女做爰猛烈叫床视频动态图| 国产成人久久AV免费高清| 少妇白浆高潮无码免费区| 免费无遮挡无码永久视频| 翁止媳庠周梦莹| 散装肉脯(h)海棠书屋| 被体育老师c了一节林妙妙| 久久久久久久精品无码AV少妇| 日日躁夜夜躁狠狠久久AV| 精品人妻系列无码人妻免费视频| 久久香蕉超碰97国产精品| 亚洲日本VA中文字幕久久|