国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1504ZR-75-30 人乳腺癌細(xì)胞

ZR-75-30 人乳腺癌細(xì)胞

簡要描述:CRL-1504 ZR-75-30 人乳腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1504
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2056

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1504  ZR-75-30 人乳腺癌細(xì)胞

ATCC® Number:CRL-1504™Price:
Designations:ZR-75-30Depositors:LW EngelBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: mammary gland; breastDisease: ductal carcinomaDerived from metastatic site: ascitesCell Type: epithelialPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-1504  ZR-75-30 人乳腺癌細(xì)胞DNA Profile (STR):Amelogenin: XCSF1PO: 10,11D13S317: 10,11D16S539: 9D5S818: 12,13D7S820: 11,12THO1: 8,9TPOX: 10,11vWA: 18,19Cytogenetic Analysis:This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 40% of cells. The rate of cells with higher ploidies was 1.2%. Sixteen marker chromosomes were found in most cells.Age:47 yearsGender:femaleEthnicity:BlackComments:ZR-75-30 was derived from malignant ascites fluid from a 47-year-old premenopausal Black woman with infiltrating ductal carcinoma. These cells have been characterized to be human, non-HeLa, malignant mammary epithelium in origin.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommendedMedium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseDoubling Time:110 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020purified DNA:ATCC 45532purified DNA:ATCC 45533purified DNA:ATCC CRL-1504DReferences:1230: Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 21219323031: Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 68822532275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764


CRL-1504  


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品乱码久久久久软件| 久久久久亚洲AV无码观看| 女人与公拘交酡免费网站| 国产精品久久久久9999赢消| 最新国产の精品合集bt7086| 大陆熟妇丰满多毛XXXX| √天堂中文官网8在线| 大香伊在人线免| 亚洲精品久久久久久久久久久| 欧美 亚洲 日韩 国产综合| 草莓视频APP黄| 亚洲欧美精品午睡沙发| 亚洲 欧美 叧类人妖| 狠狠色噜噜狠狠狠777米奇小说| 护士在办公室里被躁中文字幕| 日本爆乳强伦中文字幕电影| 亚洲女人被黑人巨大进入| 狠狠色丁香婷婷综合潮喷| 日本AAA少妇高潮免费中国| 嗯灬啊灬把腿张开灬a片| 18禁裸男晨勃露j毛免费观看| 极品少妇被猛的白浆直喷白浆| 人人爽人人澡人人高潮| 国产精品久久久久精品艾秋| 迈开腿让我看看你的草莓| 母亲とが话しています免费| 亚洲AV无码国产综合专区| 韩国无码AV片在线观看网站| 4444亚洲人成无码网在线观看| 我的真實亂倫故事| 女口述第一次放进去的感受| 欧美巨鞭大战丰满少妇| 久久婷婷五月综合色国产香蕉| 国产精品久久久亚洲| 色窝窝无码一区二区三区| 天码AV无码一区二区三区四区| 日韩午夜欧美精品一二三四区| 教子做爰xxxx| 校花张开腿疯狂娇吟k视频| 末成年娇小性色xxxxx| 亚洲成av人电影在线观看|