国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676 WM-266-4 人黑素瘤細(xì)胞

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

簡(jiǎn)要描述:CRL-1676 WM-266-4 人黑素瘤細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問  量:1973

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Designations: WM-266-4

Depositors: M Herlyn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Cellular Products: proteoglycan antigen characteristic of melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 13

D7S820: 8

THO1: 7,9

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.

Age: 58 years

Gender: female

Comments: The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma.

Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-1675



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
jk女神自慰流白浆呻吟av| 免费看真人直播| 国产成人AV乱码在线观看| 国产猛男猛女超爽免费视频| 免费看曰批女人爽的视频 | 成人女人爽到高潮的a片| 好爽又高潮了毛片免费下载| 精品人妻无码一区二区三区 | 国产大片b站免费观看推荐| 小莹乳液汁水停电了还能用吗| 亚洲最大成人网站| 久久国产欧美国日产综合抖音 | 久久伊人精品青青草原APP| 男生女生向前冲第六季| 亚洲av无码99久久久国精| 阿公抱着我边摸边吃奶视频| 在教室伦流澡到高潮h| 日韩精品一区二区三区| 阿娇被躁120分钟视频| 欧美国产在线播放欧美产品| 暴虐sm灌浣肠调教a片男男| 日产无码久久久久久精品| 亚洲熟妇色XXXXX欧美老妇Y| 多毛BGMBGMBGM胖在线| 精品人妻AV无码一区二区三区| 亚洲乱亚洲乱妇无码麻豆| 亚洲中文字幕无码爆乳AV| 久久精品人人做人人爽电影蜜月| VIDEOS日本多毛HD护士| 国产精品一区二区AV| 人妻少妇偷人精品无码| 久久精品国产亚洲AV久| 国产无套内射普通话对白| 苍井空浴缸大战猛男120分钟| 天堂MV在线MV免费MV香蕉 | 又大又紧又粉嫩18P少妇| 国模无码视频一区二区三区| 日韩精品久久久久久久电影蜜臀 | 香港三级午夜理伦三级三| 又大又粗又爽18禁免费看| 啊灬啊灬啊灬啊灬快灬高潮了|