国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>COLO-320人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡要描述:COLO-320 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:COLO-320
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2066

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO-320 人結(jié)腸腺癌細(xì)胞

ATCC® Number: CCL-220™

Designations: COLO 320DM

Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens (human)

Morphology: rounded and refractile

COLO-320 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

COLO-320



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
无码人妻AV免费一区二区三区| 国产成人精欧美精品视频| 最近免费中文字幕中文高清5 | 国产精品JIZZ在线观看老狼| 猫咪av成人永久网站在线观看| 色欲AV伊人久久大香线蕉影院| 色偷偷AV老熟女| 肥白大屁股bbwbbwhd| 99无人区码一码二码三码四码| 成人区人妻精品一区二区不卡网站| 奶头挺立呻吟高潮av全片| 国产在线拍小情侣国产拍拍偷| 日韩精品成人亚洲专区在线电影| 男男调教后菊撑开扩张a片| 亚洲和欧洲一码二码区别在| 日韩人妻高清精品专区| 77777_亚洲午夜久久多人| 野外真人实战cs| 双性受抽搐潮喷调教老师与学生| 亚洲AV无码国产精品夜色午夜 | A级毛片免费观看在线播放| 免费高清视频| 东京热加勒比高清无线| 青青久久久久精品亚洲AV中文| 亚洲AV午夜国产精品无码中文字| 污视频在线观看| 日韩在线观看视频| 曰本女人与公拘交酡| 午夜精品久久久久久久99热蜜桃 | 人妻 校园 激情 另类| 黑人巨茎大战白人女40CM| 上流社会韩国电影| 你的奶好大让老子摸摸的说说| 天堂中文在线资源库| 377人体粉嫩噜噜噜| 理论片87福利理论电影| 极品熟妇大蝴蝶20P| 亚洲人成人网站色WWW| 动漫人物桶动漫人物免费观看网站 | 女人被添荫蒂舒服极了| 国产欧美一区二区精品性色|