国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>FL62891人肝細(xì)胞

人肝細(xì)胞

簡要描述:CRL-11005 FL62891 人肝細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:FL62891
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-25
  • 訪  問  量:2157

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11005 FL62891 人肝細(xì)胞

Designations: FL 62891
Depositors: ImClone Systems Inc.
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen


Medium & Serum: San)ee PropagationATCC® Number: CRL-11005™ Price: $353.00


Growth Properties: adherent

Organism: Homo sapiens (hum

Morphology: fibroblast

CRL-11005 FL62891 人肝細(xì)胞

Source: Organ: liver

Cell Type: immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Age: fetus

Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 32.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 32°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999



CRL-11005 FL62891



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
花火视频影视大全免费观看| 亚洲欧美激情精品一区二区| 玩弄老太婆bbw视频| 第一次破了女处小红| 乳尖乱颤娇喘连连a片在线观看| 国产女人的高潮国语对白入口| 国产精品无码免费播放| 另类小说 色综合网站| 亚洲第一成人网站| 性色AV一区二区三区无码| 大山里性混乱生活| 久久久久亚洲AV无码专区桃色| 波多野中文无码av人妻系列视频| 狠狠色婷婷久久一区二区| 强壮公弄得我次次高潮厨房| 我趁老师睡觉摸她奶脱她内裤| 137肉体裸交XXXXX摄影| 苍井空与黑人90分钟全集| 久久亚洲精品中文字幕| 调教 sm 重口 h文 hy| 国产精品久久久亚洲偷窥女厕| 丰满饥渴老女人HD| 亚洲AV无码乱码在线观看| 母亲とが话しています播放| 亚洲色久悠悠av在线观看 | 亚洲精品无码久久久久秋霞| 色欲久久久天天天综合网| 亚洲国产成人无码AV在线播放| 暴躁老外玩minecraft| videossex变态狂另类| 成人在色线视频在线观看免费社区 | 亚洲成AV人片在线观看无| 久久精品久久久久久久精品| 蒙古少妇bbb多毛露屁| 亚洲成A人片在线观看中文| 疯狂的肥岳交换130部小短片| 我和公发生了性关系视频| 扒开双腿疯狂进出爽爽爽视频免费 | 孕妇滴着奶水做着爱a| 亚洲AV乱码一区二区三区按摩| 国产精品日韩欧美一区二区三区|