国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

FL62891細(xì)胞, 人肝細(xì)胞

簡(jiǎn)要描述:FL62891細(xì)胞, 人肝細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-11005
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問(wèn)  量:1974

產(chǎn)品分類

Product Category

詳細(xì)介紹

FL62891細(xì)胞, 人肝細(xì)胞

ATCC® Number:CRL-11005™    Price:$353.00
Designations:FL 62891

Depositors:ImClone Systems Inc.

Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast

Source:Organ: liver
Cell Type: immortalized with SV40 large T antigen


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
FL62891細(xì)胞, 人肝細(xì)胞

Age:fetus

Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 32.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 32°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:48 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020



References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
多人疯狂的在她体内撞np| 狠狠综合久久AV一区二区三区| 亚洲美女高潮久久久久| 国产真实的和子乱拍在线观看| 天体野营| 亚洲人色婷婷成人网站在线观看| av在线免费| 无码国产精成人午夜视频一区二区 | 97人人爽人人爽人人人片av| 精品国产偷窥一区二区| 曰本丰满熟妇XXXX性| 深灬深灬深灬深灬一点| 欧美黑人疯狂性受XXXXX喷水| 武器a原版| 欧美激情在线播放| 老卫抱着淑蓉进房间第八集视频| 天天综合天天做天天综合| 被调教的少妇雅芳1一19| 国产麻豆一精品一AV一免费| 粉嫩xb粉嫩xb粉嫩xb| 欧美大尺寸suv欧美| 69久久国产精品亚洲大片| 美妙人妇系列1~100| 18禁无遮挡羞羞污污污污免费| 不卡无码人妻一区三区音频 | 国产香蕉97碰碰久久人人| 国产不卡一区二区| 性色欲网站人妻丰满中文久久不卡| 暴躁老外玩minecraft| 麻豆国产一区二区三区四区| 久久精品无码一区二区无码| 啊灬啊别停灬用力啊黑人| 久久久久人妻精品一区三寸| 糖心VLOG精品一区二区| 寂寞少妇扒开双腿猛烈进入免费看| 国产免费午夜A无码V视频 | 99久久精品日本一区二区免费| 业余 自由 性别 成熟偷窥| 铜铜铜铜铜铜铜铜好大好深色| 欧美电影在线观看| 日本丰满人妻XXXXXHD|